目的

探讨5-氮杂-2’-脱氧胞苷(DAC)、顺铂(PDD)序贯应用对人三阴性乳腺癌细胞MDA-MB-231 体外增殖、周期及凋亡的影响。

方法

实验分4 组: 对照组、DAC 组(5 μmol/L DAC 处理)、PDD 组(15 μmol/L PDD 处理)、DAC 序贯PDD 组(2.5 μmol/L DAC 处理24 h, 再用8 μmol/L PDD 处理48 h)。 分别用MTT 法、流式细胞仪测定各组MDA-MB-231 细胞的增殖、周期及凋亡,并用金氏公式来评价两药联合效应。

结果

DAC 序贯PDD 组较DAC 组、PDD 组增殖抑制率高(P＜0.01)。 DAC 序贯PDD组48 h、72 h 的q 值分别为1.12、1.17,两药联合有增效作用。 DAC 序贯PDD 组G₁、S 期细胞减少,G₂/M 期细胞增多,DAC 序贯PDD 组较DAC 组、PDD 组细胞凋亡率高(P＜0.01)。

结论

低剂量的DAC与PDD 序贯应用可以抑制人三阴性乳腺癌MDA-MB-231 细胞增殖,促进MDA-MB-231 细胞凋亡。

Objective

To evaluate the effect of sequential use of 5-aza-2’-deoxycytidine (DAC) and cisplatin(PDD) on the proliferation in vitro, cell cycle and apoptosis of triple-negative breast cancer(TNBC)cell line MDA-MB-231.

Methods

The cells were divided into 4 groups: control group,DAC group(treated with 5 μmol/L DAC),PDD group (treated with 15 μmol/L PDD),DAC→PDD group(treated with 2.5 μmol/L DAC for 24 h and then 8 μmol/L PDD for 48 h). MTT and flow cytometry were used to determine the proliferation rate, cells cycles and cells apoptosis rate of MDA-MB-231 cells. Jin’s formula was used to evaluate the combining effect of DAC and PDD.

Results

The proliferation rate of DAC→PDD group was higher than that of the other two groups(P＜0.01).The q values of DAC→PDD group were 1.12 at 48 h,1.17 at 72 h, which indicated the synergistic effect. In DAC →PDD group, the cells in G₁ and S stage were decreased, and G₂/M cells were increased.The cell apoptosis rate of DAC→PDD group was higher than that of the other two groups(P＜0.01).

Conclusion

The sequential use of DAC and PDD at a low dosage can inhibit the proliferation and promote the apoptosis of human TNBC cells MDA-MB-231.